| PVS2处理60min、PVS3处理90min均能获得较高的恢复生长率。M9和富士在PVS3处理90min时成活率较高，均为67.33%。与中砧1号不同的是两者在PVS4处理90min时，能获得最高的恢复生长率。
3讨论
依据本研究结果，20mg·L是中砧1号试管苗离体保存培养基中的最佳蔗糖浓度。高浓度和低浓度蔗糖均可显著抑制试管苗生长。一般认为蔗糖浓度变高，培养基的渗透压增加超过了可承受的范围，养分水分吸收受阻，从而抑制生长。百合种质资源离体保存中，蔗糖浓度提高到50-90mg·L，有效抑制植株生长，试管苗可保存11个月以上。培养基中加入低浓度蔗糖，植物材料处于半饥饿状态，有效地获得减缓生长的效果，从而达到延长保存期的目的。低浓度蔗糖与高浓度蔗糖都具有抑制生长的良好效果，从节省成本考虑，采用低浓度蔗糖为宜。
培养基中添加的CCC浓度为5mg·L时中砧1号试管苗离体保存效果最佳。枇杷的种质保存中，25mg·L的CCC较合适，过高浓度的CCC对枇杷生长抑制作用明显，但会导致死亡率上升，不利于枇杷种质的保存；而在另一些植物种质保存中，CCC应用浓度则比较高，如CCC浓度为10-40mg·L时，对百合种质无抑制作用；而在葡萄种质离体保存中，CCC浓度为60-90mg·L是适宜的。
培养基中添加的ABA浓度为0.1mg·L时中砧1号试管苗离体保存效果最佳。在猕猴桃种质保存试验中，ABA的应用浓度比较低，合适浓度为0.1-0.5mg·L，有效延长继代间隔时间，保存8个月，存活率在53%以上。而另一些植物上，ABA应用浓度则比较高，在百合种质资源离体保存中，ABA浓度为1.0-3.0mg·L，对百合试管苗生长的抑制效果较好，以1.0mg·LABA最为适宜。3-10mg·L的ABA抑制枇杷植株的生长是适宜的。
本研究认为B9的抑制作用比较强烈，只有低浓度60mg·L时，可以保存中砧1号，但是80mg·L因为抑制过度而死亡。郭延平等在培养基中加入50mg·L的B9和0.1mg·L的ABA在室温下将称猴桃的试管苗保存了9个月。
因此，不同抑制剂对不同植物材料的作用效果有所差异，同一种抑制剂应用在不同的植物中，要达到抑制作用而不对植物造成伤害所需要的量也有所不同。
Sakai等认为PVS2处理苹果、梨、苜蓿等效果最佳。在香蕉种质保存中发现PVS2的效果最好，PVS1次之，而PVS3和PVS4的成活率几乎为零。本试验用PVS2、PVS3玻璃化法保存中砧1号离体茎尖，均得到较高存活率。但包埋干燥法的存活率与玻璃化法没有明显差异，而包埋干燥法茎尖没有恢复期，可以直接生长，并且一次可以处理大量茎尖，因此，包埋干燥法更适用于中砧1号的超低温保存。
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