| 4结论
在猕猴桃“金桃”品种的胚乳愈伤组织诱导过程中，2,4-D的诱导作用最显著，培养基1/2MS+1mg/L2,4-D+3mg/LBA+20g/L蔗糖是最佳的诱导培养基。从愈伤组织分化不定芽，需要一个不含2,4-D的培养过程。而在诱导不定芽分化过程中，高浓度的NAA对不定芽分化有一定抑制作用，当BA浓度为5μmol/L时，最适合不定芽的分化。同时低浓度的蔗糖（20g/L）和适当时间的暗培养（7d）能显著地促进不定芽的形成。应用流式细胞仪鉴定了胚乳愈伤组织诱导得到的植株染色体倍性。
图版：
A.初始的胚乳愈伤组织（initialcallusderivedfromendosperm）
B.增殖的胚乳愈伤组织(proliferativecallusderivedfromendosperm)
C.胚乳愈伤组织形成的不定芽(shootformedfromendospermcallus)
D.胚乳愈伤组织再生植株（plantletderivedfromendosperm）
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