| 在对山葡萄转色期果皮cDNA文库ESTs的分析中，我们发现了Ser-Thr激酶类，即丝氨酸/苏氨酸蛋白激酶，该激酶是一大类特异地催化蛋白质丝氨酸和苏氨酸残基磷酸化的激酶家族，参与多种信号传导过程，是一种胞内蛋白质，具有抗病信号传导途径。文库中还包括了大量的RPR蛋白，如谷胱甘肽-S-转移酶、锌脂蛋白、多聚半乳糖醛酸酶抑制蛋白等，同时发现了多个与花色苷代谢相关的基因，包括4-香豆酰CoA连接酶、查尔酮合酶、查尔酮异构酶、类黄酮3′,5′-羟化酶、花青素3-O-葡萄糖苷转移酶等。通过对这些基因进行克隆和序列分析，从而对掌握葡萄果实抗胁迫防御机制和调控花色苷的生物合成具有重要的意义。
cDNA文库只代表一定时期一定条件下正在表达的基因，是整个真核基因组中的少部分序列，所以构建特定时期表达的cDNA文库只能收录山葡萄果实在某一成熟期时表达的基因信息。这对于彻底了解山葡萄成熟过程中不同时期基因表达的规律，掌握相关功能基因的调控机理是远远不够的。只有构建不同成熟期，不同组织部位特异性的cDNA文库，同时结合表达序列标签，基因芯片等现代分子生物技术，才能发掘抗病，抗虫，高产等优质基因，最终为山葡萄品种的改良做出贡献。山葡萄果皮转色期cDNA文库随后测序工作的完成，将为山葡萄重要功能基因的克隆鉴定和山葡萄基因组的研究奠定基础。
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